Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.

Dettagli prodotto

• Codice: E-BC-K044-M_96T
• Marca: Elabscience
• Link: Apri link
• Stoccaggio: This product can be stored at 2~8°C for 12 months with shading light.
• Detection range: 0.12-7.0 mmol/L
• Metodo di rilevazione: Microplate reader
• Tipo di campione: serum;plasma;cell culture supernatant;tissue;cells
• Sensibilità: 0.10 mmol/L
• Area di ricerca: Glycolysis and Lipid Metabolism