Phosphodiesterase (PDEs) hydrolyzes the intracellular second messenger (cyclic adenosine phosphate or cyclic guanosine phosphate), thereby ending the biochemical effects of these second messengers.<br/>
The principle of this kit is that by hydrolyzing the substrate with total PDEs, the resulting substance has a maximum absorption peak at 405 nm. The OD value at 405 nm and the standard curve were measured to calculate the total PDEs activity in the sample.