Concanavalin A (Con A) agarose gel, is made by high-quality Con A covalently conjugated to an agarose gel, can quickly, efficiently, sensitively, and specifically bind to α-D-mannose and α-D-glucosyl residues, and can be used to separate components containing corresponding glycosylation modifications, such as cells, nuclei, or glycoproteins. The purified product obtained can be used for detection and analysis by Western-blot, mass spectrometry, etc.