The content of protein carbonyl increased after oxidation, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazine to form a reddish brown precipitate. The absorbance can be measured at 370 nm after the precipitation is dissolved. The carbonyl content can be calculated indirectly.
Protein Tyrosine Phosphatase-1B (PTP-1B) belongs to the family of protein tyrosine phosphatases, which specifically hydrolyze aromatic phosphates and negatively regulate insulin signal transduction by dephosphorylating tyrosine residues on insulin receptors or their substrates. In the dephosphorylation of PTP-1B, the phosphate group produced reacts with malachite green to form chromogenic substance, PTP-1B in samples is measured by measuring the absorbance change at 636 nm.
The product of the PC-catalyzed substrate reacts with the reducing agent. The reducing agent has a maximum absorbance at 340 nm, and the enzyme activity of PC is calculated by calculating the amount of reducing agent consumed in the reaction.
Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.
Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.
Pyruvate is an important component of the organism. It mainly participates in the metabolism of sugar and fat, and is also one of the intermediate products of carbohydrate metabolism. The detection principle of this kit is that pyruvate undergoes a series of reactions under the action of enzyme reagents, and the resulting substance can react with the color developer. The content of pyruvate in the sample is calculated by measuring the fluorescence value of excitation wavelength 535 nm and emission wavelength 587 nm.
In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm.
Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.
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