Nitric oxide (NO) has active chemical properties and is quickly metabolized into NO2- and NO3- in the body, while NO2- is further transformed into NO3-. This method uses nitrate reductase specifically to reduce NO3- to NO2-, and the concentration of NO is measured by the color depth.
Nitrite can react with chromogenic agent producing light red azo-compound. The content of nitrite can be calculated indirectly by measuring the OD value at 550 nm.
Nitrite reductase (NiR) in the sample can reduce NO2- to NO, so that the NO2- involved in the diazotization reaction to produce purplish red compounds is reduced, that is, the change of absorbance value at 540 nm can reflect the activity of the sample nitrite reductase.
NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.
Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm.
Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated indirectly by measuring the OD value at 715 nm.
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