Phenylalanine ammonia lyase (PAL) can catalyze L-phenylalanine to produce trans-cinnamic acid and ammonia, and trans-cinnamic acid has the maximum absorption peak at 290 nm. PAL activity can be calculated by measuring the increase of OD value at 290 nm.
Phenylalanine (Phe), chemically known as 2-amino-3-phenylpropionic acid, is an essential amino acid involved in a variety of biosynthetic processes. In the body, most Phe is oxidized to tyrosine by phenylalanine hydroxylase, and together with tyrosine, it synthefies important neurotransmitters and hormones, and participates in glucose metabolism and fat metabolism. Phenylalanine is catalyzed by enzyme , and the chromogenic agent has an absorption peak at 450 nm. The Phe content of the sample can be calculated by measuring the OD value of the chromogenic substance at 450 nm.
The principle is that the compounds formed by phosphoric acid and molybdate under acidic conditions will form color substances with malachite green. The depth of color is proportional to the content of phosphate. The phosphorus content can be calculated indirectly be measuring the OD value at 660 nm.
Phosphatidylcholine (PC) is an important component of eukaryotic cell membranes and a major source of second messenger diacylglycerol, phosphatidic acid, lysophosphatidic acid, and arachidonic acid, which can be further metabolized into other signaling molecules. It is widely found in a variety of foods, especially egg yolks, soy, fish, meat, and some seeds, and it can also be consumed through dietary supplements. PC provides structural stability and fluidity of cell membranes. In the liver, PC is involved in lipid synthesis and transport, helping to maintain lipid balance. As an important component of bile, PC promotes the digestion and absorption of lipid. PC supplements are used to intervene and treat liver diseases such as fatty liver disease and cirrhosis, and are used by athletes to improve physical fitness and endurance. Therefore, the detection of PC has important application value in medical diagnosis, disease management and health evaluation. The detection principle of this kit: Enzyme catalyze the PC and chromogenic agent to produce, with maximum absorption at 550 nm, and the content of PC in the sample can be calculated by measuring the OD value at 550 nm.
Phosphoenolpyruvate carboxylase (PEPC) catalyzes phosphoenolpyruvate (PEP) to produce oxaloacetic acid, which further reacts with NADH. NADH has maximum absorption at 340 nm. The activity of PEPC can be calculated by measuring the change of absorbance value at 340 nm.
Phosphofructokinase(also known as 6-phosphofructokinase; PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.
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