Revertose are sugars with reducing properties. In sugars, both monosaccharides containing free aldehyde or ketone groups and disaccharides containing free aldehyde groups are reductive. Revertose mainly include glucose, fructose, galactose, lactose, maltose, etc. Revertose are reduced to amino compounds after co-heating with 3, 5-dinitrosalicylic acid under alkaline conditions, and are reddish brown in alkaline solution. The amount of revertose is proportional to the depth of color of reddish-brown substances, so as to determine the content of revertose in samples.
In mammalian cells, S-adenosine homocysteine hydrolase (SAHH) is the only hydrolase involved in the hydrolysis of S-adenosine homocysteine (SAH) to homocysteine (Hcy). Excessive SAHH activity can hydrolyze SAH to generate excessive Hcy, thereby causing geriatric diseases such as Alzheimer's disease, hypercysteinemia, and coronary heart disease. Inhibiting SAHH activity can effectively alleviate the hydrolysis of SAH. The detection principle of this kit: The substrate is hydrolyzed by SAHH, and the product reacts with the chromogenic agent at room temperature to form a yellow colored product, which has the maximum absorption peak at 410 nm. The activity of SAHH can be calculated by detecting the change in absorbance at 410 nm.
Selenium is an essential microelements in the human body. It participates in many metabolic pathways of the human body, and has the functions of delaying aging, enhancing immunity and preventing many diseases. The intake of the selenium through daily diet. Excessive intake of selenium can cause selenium poisoning, leading to multiple organ damage, and insufficient intake can also cause a variety of diseases, such as Kashin-beck disease, etc. The detection principle of this kit: under acidic conditions, selenium is reduced to tetravalent selenium, and then reacts with the chromogenic agent to produce yellow complex, which increase the absorbance at 420 nm, and the color development intensity is proportional to the content of tetravalent selenium in the sample.
Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm. When detect tissue and cell samples, the protein concentration of the sample needs to be detected in addition (E-BC-K165, E-BC-K168, E-BC-K318 are recommended).
Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm.
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