TAL can decompose tyrosine to produce 4-coumaric acid, which has a strong absorption peak at 333 nm. Therefore, the activity of TAL can be calculated by measuring the OD value at 333 nm.
In strong acidic and heating condition, urea can react with diacetyl to form red diazine compound. The depth of color is proportional to the content of urea. Because the instability of the diacetyl, the diacetyl oxime usually react with the strong acid firstly in the reaction system to generate diacetyl, then react with urea to generate the red diazine compound. The reaction equation is as follows:
Urea can be decomposed into ammonia ion and carbon dioxide by urease. Ammonia ion can react with phenol chromogenic agent and form a blue substance in alkaline medium, and the production of the blue substance is proportional to the urea content which can be calculated with the colorimetric assay at 580 nm.
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