Uricase is an enzyme in the purine decomposition pathway, which can specifically catalyze the oxidation of uric acid into allantoin with higher solubility, thereby reducing the blood uric acid level. The detection principle of this kit is: uricase can oxidize the substrate, and the substrate has the maximum absorption peak at 293 nm. The uricase activity was calculated by measuring the OD value reduction at 293 nm.
Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes the synthesis of 1-phosphoglucose in the sample into UDP-glucose, and UDP-glucose is catalyzed by the enzyme to produce a color producing substance, which has a maximum absorption peak at about 450 nm. The enzyme activity can be calculated by the increase of OD value at 450 nm.
The protein in urine reacts with Coomassie Blue G-250 under acidic conditions to produce chromogenic substance. The reaction system changes from brownish red to blue. The blue substance produced has the maximum absorption wavelength at 585-605 nm.
The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.
Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula.
Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. VE content can be calculated by measuring the OD value at 532 nm.
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