β-Secretase 1 is an aspartic protease, the full name is β-site amyloid precursor protein cleavage enzyme 1(BACE 1). BACE 1 cleaves amyloid precursor protein (APP) to produce amyloid-β peptide (Aβ) of 40 or 42 amino acids. The deposition of Aβ in plaques and vessel walls in the brains of patients with Alzheimer's disease is widely believed to be the major contributor to the disease. The detection principle of this kit is fluorescence resonance energy transfer (FRET) method. A fluorescence donor group and a quenching group are divided at both ends of the substrate. The fluorescence energy is transferred from the fluorescence donor to the quenching group, resulting in the attenuation of the fluorescence intensity of the fluorescence donor molecule itself. When the substrate is cleaved by BACE 1, the quenching group of the substrate and the fluorescent donor are separated and the fluorescent donor is no longer quenched, the fluorescence of the fluorescent donor can be detected, so that the enzymatic activity of BACE 1 can be calculated. The fluorescence produced by this kit had a maximum excitation wavelength of 325 nm and a maximum absorption wavelength of 393 nm.
Phenol and sodium hypochlorite react with GABA to produce a blue-green product, which has maximum absorbance at 640 nm. GABA content can be calculated with the absorbance at 640 nm.
γ-Aminobutyric Acid (GABA) is a major inhibitory neurotransmitter in the mammalian central nervous system. GABA also has the function of promoting mental stability, regulating blood pressure level, improving brain vitality, increasing growth hormone secretion, accelerating ethanol metabolism and providing nutrients to nerve cells. GABA is catalyzed by enzyme, and the removed hydrogen will reduce NADP+ to NADPH, and the GABA content of the sample can be calculated by measuring the generated amount of NADPH.
γ-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405nm. The activity of γ-GT can be calculated according to the changing rate of absorbance value.
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