This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal SP. During the reaction, Universal SP in the sample or standard competes with a fixed amount of Universal SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal SP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal SP in tested samples can be calculated by comparing the OD of the samples to the standard curve.
ST2 Human ELISA Assay Kit
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal ST/5-HT. During the reaction, Universal ST/5-HT in the sample or standard competes with a fixed amount of Universal ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal ST/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal ST/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Superoxide Dismutase (SOD) Colorimetric Activity Assay Kit
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal T4. During the reaction, Universal T4 in the sample or standard competes with a fixed amount of Universal T4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal T4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal T4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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