MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which is proportional to the fluorescence intensity at the excitation wavelength of 520 nm and emission wavelength of 550 nm.
Maltase catalyze the corresponding substrate to produce monosaccharide. Monosaccharide produce hydrogen peroxide under the action of oxidase. Hydrogen peroxide react with chromogenic agent to form red product. The activity of maltase can be calculated by detection the optical density with spectrophotometer at 505 nm.
Maltose is a disaccharide formed by two molecules of glucose linked by an α-1, 4 glycosidic bond, also known as maltodisaccharide.Maltose is not only involved in the glucose metabolism in the body, but also widely used in the food and pharmaceutical industries, which is one of the important indicators of product quality control. Maltose is decomposed by enzymes to produce two molecules of glucose. Glucose reacts with the chromogenic agent to form a chromogenic substance, which can show color changes. There is a characteristic absorption peak at 505 nm, and the depth of its color is directly proportional to the content of maltose.
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