Lipoxygenase (LOX) is an iron-containing oxidoreductase found in plants and animals. The detection principle of this kit is as follows: LOX catalyzes the oxidation of arachidonic acid to produce fluorescence products, and the enzyme activity of LOX can be calculated according to the change of fluorescence value of fluorescence products at the excitation wavelength of 507 nm and the emission wavelength of 547 nm within a unit time.
Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.
Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.
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