Uric acid can be used as an antioxidant to remove peroxide, hydroxyl and oxygen free radicals, chelate and transfer metal ions, protect vascular endothelial cells from damage. Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid.
Uric Acid in protein-free filtrate reduce phosphotungstic acid to form tungsten blue, allantoin and carbon dioxide, the depth of blue color is proportional to the concentration of uric acid. Uric acid content can be calculated by measuring the OD value at 690 nm.
Uric Acid produces allantoin, CO2 and H2O2 under the action of Uricase, and H2O2 can produce red quinone imide compounds under the action of color developer, whose color depth is proportional to the H2O2 content, and has the maximum absorption at 510 nm wavelength, so as to calculate the uric acid content.
Uricase catalyzes the decomposition of uric acid into allantoin, CO2 and H2O2. Under the action of peroxidase, H2O2 oxidizes the non-fluorescent probe into the fluorescent substance. By measuring the fluorescence value of the system, the corresponding uric acid content can be calculated.
Uricase is an enzyme in the purine decomposition pathway, which can specifically catalyze the oxidation of uric acid into allantoin with higher solubility, thereby reducing the blood uric acid level. The detection principle of this kit is: uricase can oxidize the substrate, and the substrate has the maximum absorption peak at 293 nm. The uricase activity was calculated by measuring the OD value reduction at 293 nm.
Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes the synthesis of 1-phosphoglucose in the sample into UDP-glucose, and UDP-glucose is catalyzed by the enzyme to produce a color producing substance, which has a maximum absorption peak at about 450 nm. The enzyme activity can be calculated by the increase of OD value at 450 nm.
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