Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing. Fe2+ can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula.
Xanthine and hypoxanthine are important alkaloid purines that can be used to treat symptoms such as asthma relief and bronchodilation. Hypoxanthine can be oxidized by xanthine oxidase to form xanthine. Xanthine is catalyzed by xanthine oxidase to form uric acid. When xanthine oxidase is deficient, xanthine accumulates in urine and blood, inducing hyperuricemia and gout, and eventually leading to renal failure. The detection principle of this kit: Xanthine/hypoxanthine generates intermediate products under the catalysis of enzymes. Under the action of other enzymes, the intermediate products react with the probe to form fluorescent substances. By substituting the fluorescence values into the standard curve, the total content of xanthine and hypoxanthine in the sample can be calculated.
XOD can catalyze hypoxanthine to xanthine, and at the same time while producing superoxide anion free radicals. In the presence of electron acceptor and chromogenic agent, the purplish red substance can be generated, and the vitality of XOD can be calculated by measuring the production of purplish red substance.
Hypoxanthine are oxidized by xanthine oxidase (XOD) to produce xanthine and super oxygen anion, which will quickly converte to hydrogen peroxide in the system, and then, in the role of peroxidase, hydrogen peroxide can oxidize the non-fluorescent probe to fluorescent substance. By measuring the fluorescence value, the corresponding the activity of xanthine oxidase can be calculated.
Xanthine oxidase (XOD) is an enzyme that catalyzes hypoxanthine hydroxylation to xanthine and xanthine hydroxylation to uric acid, and is excreted by the kidney. Excessive production or insufficient excretion of uric acid can lead to hyperuricemia. Therefore, the development of XOD inhibitors can be used in the treatment of high uric acid and ventilation diseases. The detection principle of this kit: XOD catalyzed substrate to produce color substances at 560 nm has a maximum absorption wavelength, after adding inhibitors, the generation of color will be inhibited, according to the degree of inhibition to determine the effect of the inhibitor.
The zinc ion in the sample react with 5-Br-PADAP to produce the colored complex. The depth of color is directly proportional to the concentration of zinc ion. Zinc ion content can be calculated by measuring the OD values at 560 nm.
The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance, which is inactivated by the thermolabile nature of β-amylase, and then the enzyme activity of α-amylase is determined.
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